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KMID : 0364820180540010031
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Korean Journal of Microbiology
2018 Volume.54 No. 1 p.31 ~ p.37
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Genetic analysis of Campylobacter jejuni isolates from diarrhea patients in Gyeonggi-do
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Kim Woon-Ho
Choi Ok-Kyung
Jeong Jin-A
Park Sung-Hee
Lee Yea-Eun
Park Kwang-Hee
Yoon Mi-Hye
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Abstract
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Campylobacter jejuni is an important food-borne pathogen causing gastroenteritis in human. We isolated 208 strains of Campylobacter jejuni from 430 diarrhea patients and food employees with 17 food-poisoning outbreaks between 2014 and 2016 in Gyeonggi area. The strains were tested for genetic relationship and the genotype distribution using PFGE and multiplex-PCR typing. Among the 47 Penner-serotypes known for C. jejuni, it was identified as a genotype consisting of 35 genotypes by multiplex-PCR typing and represented 7 genotypes (HS2, HS4A, HS8, HS15, HS29, HS41, and HS53) in the selected strain. From the PFGE analysis of 11 food-poisoning outbreaks, 5 group of PFGE profile were obtained, and genetic similarity in these clusters ranged from 61.8 to 66.6%. This study examines the genetic diversity of C. jejuni that have been separated in the Gyeonggi area through various genetic analysis methods and identifies the correlation between strains in patients who have been infected with the disease in the future.
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KEYWORD
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Campylobacter jejuni, multiplex-PCR typing, PFGE
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